By Lorne T. Kirby
DNA fingerprinting is a progressive procedure that allows legislation enforcement firms, diagnostic laboratories and examine scientists to spot minute items of tissue, to figure out parentage and different organic relations relationships. this can be a learn of its applications.
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Extra info for DNA Fingerprinting: An Introduction
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7th ed. Churchill Livingstone, Edinburgh. Gyllensten U. 1989. Direct sequencing of in vitro amplified DNA. In PCR TechnologyPrinciples andApplicationsforDNAAmplification, 45-60. Erlich AH, ed. Stockton Press, New York. Gyllensten VB. 1989. PeR and DNA sequencing. BioTechniques 7:700-708. HartiDLandClarkAG. 1989. Principles ofPopulation Genetics. Sinaver Associates, Sunderland, MA. MaximAMandGilbertW. 1977. Anew method for sequencing DNA. Natl. Acad. Sci. USA 74:560-564. Murray RK, Granner DK, Mayes PA, and Rodwell VW.
Approximately a decade ago Maxim and Gilbert (1977) and F. Sanger and associates (1981) developed processes to directly sequence DNA fragments. The principles of the Sanger technique are as follows. The DNA to be sequenced is isolated, usually cleaved by a restriction enzyme, and amplified by cloning into a vector such as phage M13. This phage is favored for sequencing since it has two forms, double- and single-stranded. The double-stranded form is used as the cloning vector, and the single strand, produced from this, provides the sequencing template.
Vector Handling It is a good policy to separate the facilities for probe amplification using microorganism vectors from those for DNA analysis. Specimens contaminated by plasmid or other microorganisms can cause considerable problems in DNA profile interpretation. This separation includes glassware, pipettes, gel apparatus, and other possible sources of DNA transfer. Although duplication of minor equipment will be necessary, the use of disposable labware is one key to success of this operation.