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This quantity within the sequence "Advances in Biochemical Engineering/Biotechnology" makes a speciality of bioprocess and algae reactor expertise and apoptosis. All features of this interdisciplinary know-how, the place wisdom, tools and services are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and laptop technology are handled.
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Atkinson DE, Walton GM (1967) J Biol Chem 242:3239 17. Chapman AG, Fall L, Atkinson DE (1971) J Bacteriol 108:1072 18. Fynn GH, Davison JA (1976) J Gen Microbiol 94:68 19. Purich DL, Fromm HJ (1973) J Biol Chem 25:461 20. Martin IF, Liras P, Demain AL (1978) Biochem Biophys Res Commun 83:822 21. Mitscher LA, Martin LL, Feller DR, Martin JR, Goldstein AW (1971) Chem Commun 1541 22. Otsuka H, Mascaretti OA, Hurley LH, Floss HG (1980) J Am Chem Soc 102:6817 23. Oliver TJ, Goldstein A, Bower RR, Holper JC, Otto RH (1961) Antimicrob Agents Chemother 495 24.
5 1min - 1, respectively. Here also the energy requirements and hence the oxygen demand are comparable. 5 l m i n - 1 for the glucose feed concentration of 150 g l -~. The glucose feed concentration of 150 g 1- 1 corresponds to the highest final spectinomycin concentration of 690 units. 67gl -~ is the highest. These values indicate that the oxygen and carbon utilization during both the growth phase and the production phase is more balanced. Hence, the high spectinomycin yield is also energetically favored.
As the glucose feed concentration approaches the optimum value, the residual glucose concentration remains at the higher level for a longer duration. This indicates that near the optimum, the glucose feed is sufficient to meet the needs of maintenance and spectinomycin biosynthesis without becoming inhibitory or exhausting . However, at glucose feed concentrations both higher and lower than the optimum, the residual glucose concentration remains at the lower level for most of the production phase.