By Robert Hoffman
This quantity explores the evolution of bacterial melanoma treatment and describes the trendy concepts utilized in remedy at the present time. The chapters during this ebook conceal a large variety of subject matters similar to the advance of tumor-targeting Salmonella typhimurium A1-R, a microfluidic machine for specific quantification of the interactions among tumor-targeting micro organism and tumor tissue, non-invasive in vivo imaging of bacteria-mediated melanoma remedy utilizing bio-luminescent micro organism, how to in achieving remote-control of healing gene expression in tumor-targeting micro organism, and cell-cycle decoy of melanoma cells immune to cytotoxic medicinal drugs to drug sensitivity by means of S. typhimurium A1-R. This publication concludes with a bankruptcy at the destiny strength of bacterial treatment of melanoma. Written within the hugely winning Methods in Molecular Biology series layout, chapters contain introductions to their respective themes, lists of the required fabrics and reagents, step by step, quite simply reproducible laboratory protocols, and tips about troubleshooting and averting identified pitfalls.
Thorough and informative, Bacterial treatment of melanoma: equipment and Protocols is a beneficial source for a person who's attracted to melanoma and bacterial therapy.
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Extra info for Bacterial Therapy of Cancer: Methods and Protocols
The distribution of bacteria in each tissue was expressed as CFU/g tissue or CFU/mL blood. The results provided in Fig. 4 were similar to those for the putative macromolecular drug (Evans blue/albumin complex, Fig. 2) and the polymeric drug (PZP, Fig. 3). v. injection of bacteria, the distribution was noted mostly in the liver and spleen (Fig. 4a). At 6 h, however, the number of bacteria in the liver and spleen decreased markedly to about 1/10 of those at 1 h, whereas the numbers of bacteria in the tumor increased, approximately 80 -fold (Fig.
1c). 3. Gently stretch the head with the forefingers finger to make the esophagus extended, which creates a straight line through the neck and esophagus. 4. Keep the animal in an upright position and then measure the distance from the tip of the nose to the last rib on the left side by using the needle held next to the animal (Fig. 1c). This is the approximate distance to the stomach and the needle should not be advanced further than that distance to avoid perforating the stomach. 5. Direct the ball tip of the oral gavage needle along the roof of the mouth and toward the right side of the back of the pharynx (see Note 7), then gently pass into the esophagus and inject the 100 μl (see Note 8) bacterial inoculum (1 × 109 CFU).
Cancer Res 63: 3567–3574 Chapter 3 Oral Delivery of Tumor-Targeting Salmonella to Treat Cancer in Mice Dongping Wei and Lijun Jia Abstract Tumor-targeting bacteria have been developed as novel anticancer agents recently. To achieve their therapeutic effects, bacteria have conventionally been injected intravenously or intraperitoneally into animals or humans. Here, we describe the oral delivery of tumor-targeting Salmonella for cancer therapy in a mouse tumor model. We detail the experimental procedures for establishing a mouse tumor model, preparing bacterial culture, mouse gavage, and detection of the tumor-targeting capability of bacteria administered orally.